The Effect of Estradiol-17-beta on the Activity of Carbamoyl Phosphate:l-aspartate Carbamoyl Transferase in the Uteri of Immature Rats.

Previous investigations have indicated that, in bacteria, the rapid acceleration of the biosynthetic reactions leading to the biosynthesis of the pyrimidine nucleotides of ribonucleic acid accumulation of RNA, proteins, and phospholipids (10). The a,nd deoxyribonucleic acid proceeds as shown in Scheme 1 (1, 2). following study was undertaken to investigate the possibility Whether this is the major pat.hway for pyrimidine biosynthesis of a relationship between growth, the synthesis of RNA, and in mammalian tissue is not clear (3-5). the activity of carbamoyl phosphate : L-aspartate carbamoyl The first step in the biosynthetic pathway, the transfer of the transferase in the uterus of the immature rat stimulated by carbamoyl group of carbamoyl phosphate to L-aspartic acid to estradiol-17@. form carbamoylaspartic acid, is catalyzed by the enzyme carbamoyl phosphate : L-aspartate carbamoyl transferase. Gerhart EXPERIMENl’AL PROCEDURE